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Development of the Quantitative Real-Time PCR Assay for the
Detection of Mycobacterium xenopi. Rahizan Issa, Athirah
Abdul Sani, Nurul Akma Mohd Hassan, and Norazah Ahmad
Abstract
A quantitative real-time polymerase chain reaction
(qPCR) assay using SYBR Green I dye was developed for the detection
of the internal transcribed spacer (ITS) of 16S-23S rDNA region in Mycobacterium xenopi (M.
xenopi). The qPCR assay for the detection
of the M. xenopi DNA had a Cp at cycle 14.17 ± 0.07 and a Tm of
90.23 ± 0.03°C. As for other mycobacterial species, the Cp and Tm
were above cycle 21 and below 86°C, respectively. The Tm value of
89.47 to 90.03ºC could be used to detect M. xenopi and differentiate M. xenopi from other mycobacterial species.
Full Article
Kuat Oshakbayev, Zhangentkhan Abylaiuly, Baglan Zhumabekova,
Meruyert Gazaliyeva, Bibazhar Dukenbayeva, Pernekul Oshakbayev
Abstract
This panel study discovered the rank
of risk factors (RFs) for cardiovascular diseases (CVD) such as
arterial hypertension (AH) and coronary artery disease (CAD), and
studied the results of a weight loss program. The research included
1662 people. Results showed that the prevalence of CVD and non-cardiological
therapeutic diseases increased almost 2-3-fold in the group with
overweight. The basic and prime RF was overweight and weight loss
led to reduction of CVD symptoms as well as a decrease of tobacco,
alcohol and salt consumption, and the improvement of individual
physical capability. Weight loss was only due to fatty mass
reduction.
Full Article
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