ISSN 1556-6757







Volume 1, Issue 1, 2009


A Proposed Mechanism for OTK18 Regulation of TNF-α in Response to Neurotoxic Insult

Stephanie L. Larson, Christine E. Gilling, Kimberly A. Carlson


OTK18, a transcriptional suppressor with putative anti-retroviral properties, is found at the highest levels in severe human immunodeficiency virus type-1 encephalitic brains. The role of OTK18 up-regulation in neuronal survival and corresponding responses of tumor necrosis factor-alpha (TNF-α) and neurotrophin 3 (NT3) was examined. Rat pheochromocytoma cells were transfected with either pEGFP-OTK18 or empty vector, glutamate treated, and cell viability assessed. Quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay analyses showed significant up-regulation of TNF-α message and protein in pEGFP-OTK18 transfected cells compared to controls. These results suggest a potential role for OTK18 in regulating TNF-α and subsequent neuronal demise. Full Article

Application of the Gene-Ratio Method in the Diagnosis and Prognosis of a Variety of Human Diseases. Nathan Gaines, Christine O’Rourke, Pattle Pun, Gavin Gordon


A simple, highly accurate gene expression ratio-based methodology has been shown to be useful in the diagnosis and prognosis of cancer. This “Gene-Ratio Method” affords many advantages over similarly accurate mRNA-based assays that make it more suitable for clinical applications. It was determined that the gene ratio technique could be generally applied to other publicly available gene expression data representing a variety of normal and diseased human tissue types. This technique was able to distinguish in a statistically significant manner between normal and tumor tissues as well as among different tumor types and benign diseases using data from both cDNA and oligonucleotide microarray platforms. Full Article